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Because vitamin (especially folate) supplementation can efficiently reduce plasma homocysteine levels, further prospective cohort studies and ongoing, randomized, placebo-controlled trials will determine the efficiency of folate supplementation, possibly in combination with vitamins B 12 and B 6 , in the prevention and correction of vascular disorders related to moderate hyperhomocysteinemia

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Excess unbound probe was removed from the remaining reaction mixture (~200 g protein) using 10 kDa Amicon Ultra centrifugal filters (Merck, UFC5010) with DUB buffer containing 2% SDS

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Laboratory Tests For the diagnosis of pernicious anemia, the following laboratory tests are frequently used