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DNA-binding probes DAPI (4,6-diamidino-2-phenylindole dihydrochloride), Alexa Fluor 546-Phalloidin and Alexa Fluor 488 and 546-goat anti-rabbit IgG antibodies were from Thermo Fisher Scientific (USA)

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Tregs themselves are vital in peripheral immune tolerance and suppressing autoimmunity and inflammation through control of adaptive and innate immune responses (Astarita et al., 2023)

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