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Mechanistically, GHRH-receptor engagement activates the cAMP pathway adenylyl cyclase, cAMP, and protein kinase A (PKA) in sequence, as outlined by Culhane et al
[DOI] [PMC free article] [PubMed] [Google Scholar] 92.Klingberg E., Olerod G., Konar J., Petzold M., Hammarsten O
It's a slow path to degradation but a certain one
PLoS ONE 9 , e112811 (2014)
We note that this allele has a small in-frame deletion that removes two amino acids, P447 and I448 as well as the ZF1 degron, but has the same GFP expression pattern as EPN-1 ::GFP transgenes and no lethality or any other overt defects suggesting that epn-1 activity is not significantly compromised
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