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To a 50-l aliquot of sample or MDA standard, 50 l of 0.05% butylated hydroxytoluene (BHT), 400 l of 0.44 M H 3 PO 4 , and 100 l of 0.42 mM 2-thiobarbituric acid (TBA) were added and heated for 1 h at 100C, followed by 250 l n -butanol extraction of the MDATBA derivative

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Therefore, we aimed to demonstrate whether the protective effect of daphnetin is achieved via GLP-1R-mediated oxidative stress and inflammation