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The peptide was synthesized using solid-phase methods and purified via reverse-phase high-performance liquid chromatography (HPLC)

From a pharmacokinetic perspective, topical GHK-Cu has a distinct advantage: it acts locally rather than systemically

Once the lysate had been loaded, the column was washed with 20 column volumes (CV) wash buffer, and the His-tagged TRFH domain was eluted using a linear gradient from 0 to 100% elution buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 300 mM imidazole) over 25 CV

Modulating skin colour: role of the thioredoxin and glutathione systems in regulating melanogenesis

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