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The precipitate was collected (6000 rpm, 30 min, 4 C) and washed with 1 mL of acetone three times to remove the TCA

This article is a science-first exploration of five peptide families with the most robust evidence bases for endurance-relevant physiology: collagen peptides (the only class with extensive human RCT data), MOTS-c (the mitochondrial exercise mimetic), BPC-157 (the gastric cytoprotective peptide), TB-500 / Thymosin Beta-4 (the actin-regulating wound healer), and GHK-Cu (the copper-binding tissue remodeler)

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The conditioner you use can also make a difference, but finding the right products may take some trial and error

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