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Subsequently, fungal cells were stained with Calcofluor White (Sigma-Aldrich) at a final concentration of 1 g/ml and Sytox Orange (ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 M for 10 min in the dark
In addition, beyond lipid digestion, bile acids act as key signaling molecules that regulate hepatic metabolism, gut microbiota, intestinal integrity, and immune as well as glucose and lipid homeostasis through receptors such as the farnesoid X receptor and Takeda-G-protein-receptor-5 [75]