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FDA-approved peptides (Saxenda, Wegovy, Zepbound) cause gastrointestinal side effects like nausea, vomiting and diarrhea in up to 80% of patients, with rare serious events including gallstones or inflammation of the pancreas

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However, when tirzepatide was later approved for obesity management in late 2023, it opened the door for Eli Lilly to potentially gain an additional 3 years of exclusivity for this new indication

SNAP/FLAG-tagged human GLP-1Rs (SNAP/FLAG-hGLP-1Rs) with single alanine substitutions for each selected residue were successfully expressed in INS-1 832/3 GLP-1R KO cells with no significant differences detected in cell surface expression levels versus WT GLP-1R (Figure 2H, top graph)