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For long-term storage beyond 28 days, aliquot the reconstituted solution into smaller sterile vials (0.10.2mL each) and freeze at 20C

Two major examples: Hypothalamic-pituitary-thyroid (HPT) axis : Hypothalamus releases TRH anterior pituitary releases TSH thyroid gland releases T3/T4 regulates metabolism Hypothalamic-pituitary-adrenal (HPA) axis : Hypothalamus releases CRH anterior pituitary releases ACTH adrenal cortex releases cortisol regulates stress response Feedback loops are what keep these axes in balance: Negative feedback is the most common mechanism

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