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The mobile phase was solvent A (10 mM ammonium acetate and 0.2% formic acid in water) and solvent B (10 mM ammonium acetate and 0.2% formic acid in 95% HPLC-grade acetonitrile/5% HPLC-grade water)

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The body was adapting to the new metabolic state, and participants were approaching their natural plateau points