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This population was the primary focus of the SURMOUNT trials and tends to see the most dramatic absolute weight loss

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However, sustained inflammation promotes BBB disruption by inducing microglial-led phagocytosis of astrocytic end-feet [62]

Loading GST-fusion proteins onto glutathione beads steps: Now that you have your protein of interest bound to the glutathione agarose beads through GST, youll want to add the potential interacting proteins to this mixture