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Immunoprecipitated samples were washed four times with 1 ml of NET-2 buffer and stored in 10 mM Tris-HCl (pH=8.1) at 80 C

The results revealed a significant reduction in BW, WAT weight, and adipocyte size in epididymal WAT (eWAT), as well as improved insulin sensitivity in mice fed with M-HFHSD during the active phase, but not during the rest phase