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The resting membrane potential was monitored for at least 5 min (basal), followed by the addition of pGH to the bath (5 g/mL) for approximately 5 min
After 30 min of staining, cell viability was visualized and imaged under a fluorescence inverted microscope, and the numbers of live and dead cells were quantified using ImageJ software
The various forms of GPXs show a scattered picture: GPX2 and GPX4 are less affected by Se deficiency than GPX1 and GPX3 (Sunde, 2012)
In some cases, recovery might take longer than expected
After first use, most can stay at room temperature for a limited period, but "room temperature" does not mean the interior of a hot car crossing back through Tijuana in August
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