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Generation and quantification of persulfides on Cofilin1 Purified full-length human recombinant Cofilin 1 protein expressed in Escherichia coli (Abcam #62958) (11 g/sample) was incubated with GSH (1 mM), MitoPerSulf (100 M) or both in 50 mM HEPES buffer (pH 7.4) for 7 min at RT, followed by alkylation with IAM-TPP (20 mM) at RT for 30 min in the dark

[DOI] [PubMed] [Google Scholar] 22.Richards P., Parker H.E., Adriaenssens A.E., Hodgson J.M., Cork S.C., Trapp S., Gribble F.M., Reimann F

Indeed, rapamycin, an inhibitor of mTOR, suppressed the amino acid-induced upregulation of p-STAT3-S727 and decreased VGF levels (Fig