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Samples were incubated on ice for 35 min, centrifuged for 20 min at 4 C at 12,000 rpm, and protein concentrations were quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) according to the manufacturers microplate procedure protocol

Notably, similar inhibitions were observed both with the wild type enzyme and its cysteine mutants, suggesting that thiol conjugation is dispensable for GST inactivation by auronofin in contrast to other described targets 103

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doi: 10.3389/FVETS.2022.923013/BIBTEX 20 DandrieuxJRS

These effects are mediated in part by stress-responsive transcription factors that coordinate the expression of genes involved in GSH metabolism and redox control