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ABX-only) Design Type (ABX-only): A low-biomass background was established using an antibiotic cocktail in drinking water for 4 weeks to observe THH efficacy under microbiota-depleted conditions (Zheng et al., 2023) Results showed that the ameliorative effects of THH on joint swelling, inflammatory response, and bile acid metabolism abnormalities were significantly weakened or abolished after microbiota depletion (Zheng et al., 2023)