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Our study has important implications for our understanding of enzymatically active and inactive glutaredoxins and could be useful for the design and optimization of artificial glutaredoxins, glutaredoxin-coupled fluorescent redox sensors and transition-state inhibitors

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Notably, the G/D-CuP group demonstrated the lowest CD86/CD206 ratio, further indicating a promoted transition from the M1 to M2 phenotype (Supplementary Fig

The concentrated protein was applied onto a Superdex 200 Increase 10/300 GL column and eluted with buffer T containing 300 mM KCl