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The resulting residue was dissolved in 200 L 0.01 mol/L hydrochloric acid, filtered through a 0.22 m nylon syringe filter, and injected 20 L into the HPLC system for analysis

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Both define the 0.9% concentration, the pH range of 4.5 to 7.0, and the 28-day post-puncture window used here

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