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First, it makes the peptide measurable, because you cannot pipette a dry film accurately

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The role of IGF-II should be viewed in the light of Efstradiadis series of knock-out experiments in mice (21) where the Igf2 ko mice had the same degree of fetal growth retardation as the igf1 ko which demonstrates that IGF-II is a paracrine/autocrine and not an endocrine hormone

Additional Information How to cite this article : Chen, Y

Media was replaced every day and after three days the cells were fixed with methanol: acetic acid (3:1) and stained with crystal violet