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To quantify protein levels in CD8 + T cells, single-cell suspensions were stained using the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Thermo Fisher Scientific, 10119), followed by surface and intracellular staining with fluorochrome-labeled antibodies (Supplementary Table 1)

Biol Psychiat 71(11):956

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Like maternal health conditions such as preeclampsia, lack of understanding of the conditions and their multiple clinical symptoms render diagnosis complex

After incubation at 37 C for 6 h, the medium was replaced with incubation medium (Williams Medium E without phenol red supplemented with Hepatocyte Maintenance Supplement Pack)