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Therefore, the subsequent experiments were used to determine whether Sirt1 affected the nuclear translocation of Gli-1 and the expression levels of components of the Shh signaling pathway, such as Shh, Ptc-1, Smo and Gli-1

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Glutathione-responsive nanosystems are engineered to remain stable in circulation but undergo structural changes or drug release specifically in high-GSH tumor cells, improving therapeutic precision and reducing off-target toxicity

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