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As we age, NAD levels naturally decline, which has been associated with various age-related health issues, including metabolic disorders, neurodegenerative disease, and reduced cellular repair mechanisms
However, if you are performing a sensitive cell viability assay or working with very high volumes of reconstituted solution per experiment, it is worth calculating the final benzyl alcohol concentration in the experimental medium and comparing to known cytotoxicity thresholds (generally above 0.1% in culture)
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