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A double stainless steel guide cannula (26 gauge in diameter, Phymep, France) was implanted into the PVN through stereotactic procedures (A/P: 0.9 mm from bregma, M/L: 0.3 mm and D/V: 4.8 mm from dura mater) as described above

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Minor batch-to-batch variations in residual trifluoroacetic acid (TFA) saltseven within a compliant 99% purity rangecan subtly alter baseline cellular cell cultures or animal weight readings

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