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doi:10.33549/physiolres.931861

Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

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There was no statistically significant interaction between the effects of water volume and post-dose fasting period for AUC 024h,semaglutide,day10 and C max,semaglutide,day10 ( p = 0.530 and p = 0.394, respectively)