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Use bacteriostatic water for reconstitution, not sterile water, because the benzyl alcohol preservative in bacteriostatic water inhibits microbial growth in the vial throughout the usage period

Gluten sensitive enteropathy, for example, results in inflammation of the bowel surface, which reduces the bowels absorption of a variety of nutrients (including iron)

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For immediate use in cell culture, direct reconstitution in buffer is fine and sometimes preferred