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The suspension was then ultracentrifuged for another hour at 41,000 rpm at 4C to separate detergent-resistant (DRM) from detergent-soluble (DSM) membrane fractions: the disordered DSM fraction in the supernatant was retained for analysis and the ordered DRM (lipid raft) fraction was resuspended in 1% SDS plus protease and phosphatase inhibitor cocktails, sonicated, centrifuged for 10 minutes at 13,500 rpm at 4C, and analysed via SDS-PAGE and Western blotting as in (25)
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There were no unexpected safety findings at the Phase 2 stage that would fundamentally alter the risk-benefit profile expected for this class
Rights and permissions About this article Cite this article Hasanuzzaman, M., Nahar, K., Anee, T.I
Its easy to get carried away imagining hordes of young people shooting up unknown substances in gym room toilet stalls, but thats probably the extreme end of the picture
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