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MOTS-c (New England Peptide, USA) was administered daily at 5 or 15 mg/kg via intraperitoneal injections

Future studies should cover populations of different ages, genders, and regions to fully assess the range of safe doses of chili peppers in different populations and their ongoing effects on ADHD symptoms

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The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol