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Identifying robust biomarkers, such as GPX4 expression, lipid peroxidation signatures, or iron metabolism markers, will be essential for patient stratification and therapeutic optimisation

Western blotting For western blotting experiments, tissues were lysed with Magnalyser beads in radioimmuno-precipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1% SDS, 0.5% Nadeoxycholate, 1% NP-40, adjusted to pH 7.27.4) supplemented with PIC (complete Tablets EASYpack, Roche, Basel, Switzerland) and PhosStop (Roche, Basel, Switzerland)

Several independent studies have reproduced the initial results of Sikiric's team regarding gastric ulcer and tendon injury healing, strengthening the credibility of these observations

When did the problem develop

Body composition changes typically require 4 to 8 weeks of consistent supplementation combined with diet and training