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Here is a comprehensive breakdown: DSIP: The available clinical studies regarding the effects of DSIP on sleep do not report any side effects associated with its use [3]

A dual agonist adds a GIP arm that further inhibits appetite-driving neurons, and a triple agonist such as retatrutide adds a glucagon arm that raises energy expenditure

Acetyl-L-carnitine confers neuroprotection against lipopolysaccharide (LPS) -induced neuroinflammation by targeting TLR4/NFB, autophagy, inflammation and oxidative stress

The sections are then rehydrated, leading up to the H&E staining where hematoxylin stains the nuclei blue and eosin stains the cytoplasm and extracellular matrix pink