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An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

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On the contrary, increasing the expression of GPX1 will bring protective effects to the corresponding tissues and organs

These compounds are vital to keep our cells healthy, though they work differently

[13] Thirdly, tyrosinase inhibition is effected indirectly via its antioxidant effect