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Samples were reconstituted in 100 L and 2 L of the sample was injected from a 4 C autosampler into a ZIC-pHILIC 150 2.1 mm 5 m particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific) at 25 C

It has a melanin synthesis inhibitory effect due to competition with L-DOPA in binding to tyrosinase during melanin synthesis

12 weeks: Roughly 69 mL used

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