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After overnight culture, transfected cells were incubated with 200 g mL 1 D-luciferin (BioVision) in an incubator at 37 C and 5% CO 2 for 1 h
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The grid points need to be horizontally arranged in a straight line 1 cm below the gluteal fold
Therefore, HEK293 lysates were incubated with the N -benzylamide-derived alkyne probes (Supplementary Fig
GCG can suppress circulating amino-acids [59] and increase protein catabolism, mechanisms thought to contribute to elevated energy expenditure and WL [32, 33, 60]
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