After the treatment, the culture medium was replaced with 70 L of cold 1% Triton-X-100 for cell lysis, incubated for 10 min and centrifuged (4800 g
As expected, CNS-administered leptin and MTII resulted in significant decreases in body weight of the rats throughout the 4-week treatment period (see Figure 1C in Gava et al
doi: 10.1017/S0007114510003363 201 RobertsA
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