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doi: 10.1016/j.carres.2008.09.024 9
The empty stomach rule is the easiest and most critical protocol for researchers to follow for consistent timing.
The tissue was homogenized thrice at 1500 rpm for 120 s using a tissue homogenizer (Micro Smash MS100R, Tomy Digital Biology Co., Ltd., Tokyo, Japan) following which the homogenate was centrifuged at 2300 g and 4 C for 5 min
IGF-1 is typically composed of 70 amino acids but IGF-1 DES is a shortened analogue with only 67 amino acids
A recent effort to systematically uncover the scope of 20S proteasome substrates, adopted a distinctive approach by preconditioning HeLa nuclear-rich cellular extracts at 95 C before subjecting them to degradation by purified 20S proteasomes (Myers et al, 2018)
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