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No degradation of protein was seen in an SDS-PAGE gel after 2, 5 or 7 days

The eluted yeast crude proteins were used for the enzymatic activity assay described below

doi: 10.3389/fnmol.2019.00109 69 NardiLChhabraSLeukelPKrueger-BurgDSommerCJSchmeisserMJ

This movement enables an increased pool of GSSG in the cell for potential use by specific glutathione enzymes like GST, emphasizing the importance of glutathione-associated enzymes as key molecules in the resistant response

Run order of samples was randomized and in lieu of internal standards, technical replicates were injected every 8 samples to assess quality control and instrument performance