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Single cell suspensions of LN cells were prepared by gentle mechanical disaggregation through 200 micron-mesh stainless steel gauze and resuspended in RPMI-1640 (Life technologies corporation, Grand Island, NY, USA) supplemented with fetal calf serum (Life technologies corporation, Grand Island, NY, USA) (10%), HEPES (Sigma-Aldrich, St

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Go AS, Chertow GM, Fan D, McCulloch CE, Hsu CY

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