purification of proteins fused to glutathione s-transferase GST-Tag Schematic outline of the GST-His

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Increased apoptosis and necroptosis in the aortic wall

The assay typically involves the generation of nitric oxide from sodium nitroprusside in an aqueous solution, where the interaction of nitric oxide with oxygen leads to the formation of nitrite ions, which can be quantified using the Griess reagent (Prior et al., 2005

J Chromatogr B 861:123129

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