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In recent years, with the deepening of the research on brain diseases, the exploration and application of catalpol in the therapy of brain diseases have gradually increased

Safety profiles differ rather than one being universally safer

This requires a prescription and medical supervision, and is typically reserved for hospitalized patients or high-risk outpatients

After washing two times with M9 buffer (3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , 5 g NaCl, 1 ml 1 M MgSO 4 , H 2 O to 1 litre), whole worms were fixed with 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 20 min, and then post-fixed with cold 100% methanol for 5 min at 20C (alternatively, fixed worms can be stored in cold methanol at -20C for a few days)

Prevention: Use low dead-space syringes designed to minimize waste