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The vector for protein production, pJB-GST-HTS-HS-GFP, was created by first cloning a 7 amino acid spacer fragment containing an XhoI site into pET-15b (Novagen) using sticky ligation and BamHI/XhoI sites

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The compound's selectivity profile minimizes concerns about off-target effects, but investigators should consider potential interactions with other NAD+-modulating interventions or compounds that affect methylation metabolism

Visualisation and clustering of scRNAseq was performed in Scanpy 27 following standard quality checks (empty barcodes, percentage of mitochondrial genes)

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