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This is relevant because metabolic dysfunction-associated steatotic liver disease (MASLD, formerly NAFLD) is extremely common in people with obesity and is difficult to treat with conventional approaches

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The pellet was subsequently washed with 10 ml of the G-solution, which comprised of 500 ml HBSS (BE10-508F, Life Technologies) with 10% BSA (126615, Sigma Aldrich) and 1% Pen-Strep (15140122, Life Technologies), and re-suspended in 5.5 ml of gradient solution (15% Optiprep (5 ml 10% RPMI, Life Technologies) + 3 ml of 40% Optiprep, which was diluted from 60 % Optiprep with G-solution (D1556, Sigma Aldrich)) per sample, and placed on top of 2.5 ml of the gradient solution

Massage area As the fat travels through the lymphatic system, the area needs to be lightly massaged every day to help the fat drain properly through the body

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