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Description

As a broad inhibitor of the PKC family, CHE significantly decreased cell differentiation in the human promyelocytic leukemia (HL-60) cell line, which is induced by the combination of TPA and capsaicin (Zheng et al., 2005)

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[PMC free article: PMC2882209] [PubMed: 16130027] 78

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Plates were subsequently washed with 0.1% polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline (TTBS), immediately fixed with cold 3.7% paraformaldehyde in PBS, and re-treated with the blocking solution overnight at 4C

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