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Islets were isolated as described previously [7] by pancreas disaggregation with an infusion of 0.7 mg/ml collagenase P containing DNAse I in HBSS without Ca 2+ /Mg 2+ following a 9 min incubation at 37C

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In addition to its other activity, tretinoin prevents hydroquinone oxidation and improves epidermal penetration of hydroquinone