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After three 15-minute washes with phosphate-buffered saline with tween-20 (PBST), the membranes were incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (1:2000 dilution, ab205718, Abcam, Shanghai, China) for 2 hours at room temperature

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Acetylcysteine also has anti-oxidant properties by reacting with free radicals and also by serving as a precursor to glutathione, which is an important intra and extra-cellular antioxidant

Recent research conducted by [16] suggests a significant association between higher ergothioneine levels and reduced mortality, as well as a lower risk of cardiovascular disease