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Finally, we discuss limitations associated with oncolytic virotherapy at the conclusion of this review

Dominant negative G s (DNG s ) in pFastBac vector, and poly-his tagged G1 and G2 in a single pVL dual vector construct were used as previously described

Even though the preservative inhibits bacterial growth, it doesnt guarantee sterility if grossly contaminated, so proper handling is essential (wiping the rubber stopper with alcohol each time, using new sterile needles, etc.)