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Calculate the percentage of l-carnitine derivative (R ) from the derivatized Sample solution: Result = r L /(r L + r D ) 100 r L = peak response of the l-carnitine derivative from the derivatized Sample solution r D = peak response of the d-carnitine derivative from the derivatized Sample solution Calculate the corrected percentage of l-carnitine (C 7 H 15 NO 3 ) (C ) in the portion of Levocarnitine taken: Result = (R L P B )/(P A P B ) 100 R L = percentage of l-carnitine derivative, calculated previously P A = percentage of ()-FLEC as determined for (+)-1-(9-Fluorenyl)ethyl chloroformate solution P B = percentage of (+)-FLEC as determined for (+)-1-(9-Fluorenyl)ethyl chloroformate solution Calculate the corrected percentage of d-carnitine in the portion of Levocarnitine taken: Result = 100 C L C L = corrected percentage of l-carnitine, calculated previously Acceptance criteria: NMT 0.2% of d-carnitine Limit of Potassium [NoteThe Standard solution and the Sample solutions may be modified, if necessary, to obtain solutions of suitable concentrations adaptable to the linear or working range of the instrument.] Standard solution: 31.25 g/mL of potassium in water, prepared from potassium chloride previously dried at 105 for 2 h Sample stock solution: 0.625 mg/mL of Levocarnitine in water Sample solution A: Transfer 20.0 mL of the Sample stock solution to a 25-mL volumetric flask, and dilute with water to volume

Reduced glutathione (GSH), which is employed by Gpx4, was increased in CDAHFD but unaffected by Gpx4 Tg/+ (Fig

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