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Cells were washed three times with 1 ml PBS and lysed with 200 l 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 C for 5 min, followed by the addition of 800 l PBS

The % Difference in % D-Carnitine content between Method Precision and Intermediate Precision met the acceptance criteria of not more than 10, and hence, the method is rugged ( Table 6 )

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