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Diabetologia 39 , 12271232 (1996)

Tissue Health During Weight Loss Significant weight loss involves metabolic and structural remodeling throughout the body

doi:10.1016/j.molcel.2013.01.018 3

To examine whether BPM31510-dependent impairment of substrate-driven respiration results in concomitant electron leakage and ROS production, a method was established to measure substrate-dependent H 2 O 2 production by initiating respiration with specific substrates and kinetically quantifying H 2 O 2 production using Amplex Red reagent (Supplemental Fig