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In addition to the primary enzymes and intermediates of the folate cycle, the N 5 -formimino-THF (5-forminino-THF) that is produced in the context of histidine catabolism can also contribute to the folate cycle

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Waters) and separated using ultra HPLC on an Accela 3000 UHPLC (Thermo Fisher Scientific) using gradient elution at 0.5 ml min 1 and column temperature 55 C

Finally, this treatment reduced the levels of the pro-inflammatory cytokines IL-1 and IL-33 while maintaining anti-inflammatory cytokine IL-10 levels, and maintained the levels of endogenous antioxidants in the colons of colitic mice (173)( Supplementary Table 1 )