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If deproteinization of plasma prior to storage is necessary for prevention of spontaneous oxidation, then there would have been no difference in levels of GSH between Preparation Condition 1 and Preparation Condition 2, and we would have found no difference in level of total GSH and differences in levels of free GSH, GSSG, and their relative ratio between Preparation Condition 3 and Preparation Condition 4

2.2 Mechanism of ferroptosis occurrence 2.2.1 Core regulatory pathways 2.2.1.1 System xc /GSH/GPX4 signaling as primary regulatory hub Ferroptosis regulation is widely recognized as primarily involving the constitutive inhibition of system Xc , which triggers a cascade leading to glutathione (GSH) exhaustion and GPX4 functional impairment

Rossi, Effects of hydrolyzed collagen supplementation on skin aging: a systematic review and meta-analysis

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