Semaglutide is newly approved for weight loss, but it has been approved and used to treat diabetes since 2017
To evaluate growth on solid media, in all cases -with the exception of the metE mutant, which only grew in the presence of B12 and, therefore, plating with and without B12 was performed from liquid cultures supplemented with B12- the liquid cultures grown without B12 were plated in solid 7H10-ADC plates supplemented with or without AdoCbl (10 g/mL) and/or L-Met (25 g/mL)
L., Joshy, G., Lewington, S., Manson, J
Instead, gently swirling or tilting the vial ensures the solution mixes evenly without compromising the peptides stability and efficacy
[PubMed] [Google Scholar] 73.Campbell JE